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Figure 1


Fig. 1. In vitro binding between N-APC fragments and full-length C-APC. (A) Schematic of full-length APC (aa 1-2843), and the position and exact residues contained in the fragments that were used in this study. (B) Autoradiogram of His-tagged N1, N2 and N3 APC fragments that were generated using an in vitro translation system in the presence of 35S-methionine. (C) Coomassie gel and immunoblot of purified His-tagged C-APC, C3 and N3 using an anti-His-tag antibody. (D) Purified C-APC was incubated with in vitro translation reaction products for N1, N2 and N3. C-APC was immunoprecipitated with a polyclonal anti-C-APC antibody and bound N-APC fragments were detected by autoradiography. For control samples (Ctrl), the purified C-APC protein was omitted. To confirm that equal amounts of C-APC were used in each case, the same samples were immunoblotted with a monoclonal anti-C-APC antibody.





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