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Fig. 6. Clusters formed by APC ${Delta}$ N3 are less dynamic. (A-C) Images of clusters containing GFP-APC (A), GFP-APC ${Delta}$ N3 (B) or GFP-APC ${Delta}$ C3 (C) were recorded every 100 seconds to monitor shape and intensity changes over time. The summed intensity in each cluster was recorded and two examples of different types of clusters at 0, 300, 600 and 900 seconds are shown. Please note that these images show projections of the total summed intensity, not maximum intensity projections. This makes their appearance more fuzzy, but allows the changes in total intensity to be quantitated and visualised accurately. The appearance of clusters formed by GFP-APC changed noticeably more than of those formed by GFP-APC ${Delta}$ N3, whereas changes by those formed by GFP-APC ${Delta}$ C3 were intermediate. (D) Total fluorescent intensity in selected clusters was measured and recorded over time (seconds). The intensity in each cluster relative to that at the start is plotted for each time point to show that clusters formed by GFP-APC ${Delta}$ N3 are less dynamics than those formed by GFP-APC or GFP-APC ${Delta}$ C3.

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