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Fig. 3. The distribution of Yuri isoforms throughout development. Immunoblots for Yuri isoforms are shown. (A) Specificity of Yuri antibodies. Lane 1, 30 unfertilized eggs from Df(2L)do1/CyO-GFP mothers [Df(2L)do1 removes yuri]. Lane 2, 30 terminal homozygous Df(2L)do1 embryos. Lane 3, 30 terminal homozygous CyO-GFP (homozygous yuri⁺) embryos. The two large isoforms are not present in unfertilized eggs or embryos lacking yuri, but are zygotically expressed in the yuri⁺ embryos. (B) Yuri isoforms during embryogenesis. The larger Yuri isoforms appear late in embryogenesis in embryos from control (w¹¹¹⁸) and yuri^F64 mothers mated to w¹¹¹⁸ males. (C) Yuri isoforms present in various tissues and stages. Samples from w¹¹¹⁸ control and yuri^F64 animals. Sample sizes: ovaries, 8 pairs; testes, 7.5 pairs; heads, 3; thoraces, 0.5; third instar larvae, 0.5. Bands that might be degradation products are marked with an asterisk.

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