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Fig. 4. Sperm elongate but show individualization and coiling defects in yuri^F64. (A) Sperm tails, marked with Don Juan-GFP (green), fill the seminal vesicle (arrow) in control testes. (B) In yuri^F64 hemizygotes [yuri^F64/Df(2L)do1], the seminal vesicle (arrow) is empty, and sperm cysts show abortive coiling in the testis proper (arrowhead). (A'-B'') Phalloidin staining (red) identifies actin cones and waste bags in control testis (A', red arrow; as shown at higher magnification in A''). Mutant testis is devoid of these structures (B'), and F-actin sleeves are present instead (B', red arrow; as shown at higher magnification in B''). Scale bars: 200 µm.

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