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Figure 8


Fig. 8. yuriF64 effects on the dense complex and basal body. (A-C) In the yuriF64 mutant, the Yuri nuclear stripe and dot are lost (A), {gamma}-tubulin is no longer associated with the nuclei (B) and F-actin is no longer present on nuclei (C). (D,E) In Dynein light chain mutant Dlc90F05090, Yuri association with the nuclear cap (D) and stripe (E) is diminished (arrowheads), but the bell-shaped dot of Yuri (arrows) now appears precociously on round spermatid nuclei (D). In addition, a second dot (*) of Yuri is now found at the apex of both round (D) and elongate (E) nuclei. {gamma}-tubulin staining (F) reveals that this dot (arrow) is the region of the basal body distal to the CA. Scale bars: 20 µm in A-C, 10 µm in D-F.





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