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Figure 9


Fig. 9. Basal body positioning and aberrant nuclear migration in yuriF64. (A) In yuriF64 heterozygotes, GFP-PACT fluorescence reveals basal bodies clustered tightly at apical nuclear tips. GFP-PACT is lost in the final stages of nuclear condensation (arrowhead). (B) In yuriF64 homozygotes, the basal bodies are disarrayed with some positioned at the rostral nuclear tip (arrows). The most condensed nuclei again show no GFP-PACT fluorescence (arrowheads). (C,D) In both yuriF64 heterozygotes (C) and homozygotes (D), subsets of nuclei sometimes migrate to the apical end of the cyst (arrows). Asterisks and white arrow indicate the position and direction of the stem cell tip, respectively. Scale bars: 20 µm.





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