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Figure 1


Fig. 1. Microtubule cytoskeleton is required for spindle alignment. (A) Scheme of a S. pombe cell with a misaligned spindle. The angle is that formed between the mitotic spindle axis and the long axis of the cell (both axes are indicated with arrows). (B) Percentage of wild-type haploid and diploid control cells, MBC (25 µg/ml) or Latrunculin A (50 µM) treated cells, and mto1{Delta} and tip1{Delta} cells, showing the angle of the mitotic spindle relative to the long axis of the cell. The range of mitotic spindle angles of mto1{Delta} cells MBC-treated cells and tip1{Delta} cells were significantly different to wild-type cells (P<0.001) but no significant difference from the wild type was seen in Latrunculin-A-treated cells (P<0.1) or diploid cells (P<0.2).





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