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Figure 2


Fig. 2. Microtubules and microtubule-SPB attachment are required for proper spindle alignment. (A) Scheme showing the SPB trajectory from position A at time 0 to position B at time 1. (B) Overlaid images showing SPB position during a window of time ranging from 25 to 28 minutes in wild-type control cells, MBC (25 µg/ml) or Latrunculin A (50 µM) treated cells, and mto1{Delta} cells expressing the SPB marker Sid2-GFP. Arrowheads denote the overlaid SPB trajectory. The asterisk denotes a cell in mitosis. Dashed lines indicate the cell outline. (C) Overlaid images showing the SPB position during the indicated time interval followed by images taken one or two minutes before SPB separation in wild-type control cells, MBC-treated cells (25 µg/ml), mto1{Delta} cells, and the cell morphology mutants tea1{Delta} and orb6-25, expressing sid2-GFP. Arrowheads indicate the initial SPB separation.





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