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Figure 6


Fig. 6. Effect of adhesion on RhoG activity. (A) MEF cell lysate was loaded with GDP or GTP{gamma}S as described in the Materials and Methods, and binding of GTP-RhoG to GST-ELMO was measured. (B) Left panel: MEFs were replated on fibronectin-coated dishes for the indicated times and RhoG activity was assayed using the ELMO pulldown assay. Transient expression of the first Trio DH-PH domain was used as a control for activated RhoG. Right panel: quantification of RhoG pulldown assay normalized to total RhoG. Values are means ± s.d.; n=3.





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