spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. The TMD of Fis1 is sufficient for mitochondrial targeting. (A) Schematic representation of the GFP-Fis1(TM) protein. (B) Mitochondria (M) were purified via sucrose gradient from wild-type yeast cells or from cells transformed with a vector encoding GFP-Fis1(TM). The post mitochondrial fraction was further separated by differential centrifugation into ER fraction and cytosol (C). Proteins were subjected to SDS-PAGE and immunocytochemistry with antibodies against the GFP moiety, a control marker protein for the cytosol (Bmh1), a control ER marker protein (Erv2) and the mitochondrial outer-membrane protein Tom20.





Right arrow Return to article