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Figure 6


Fig. 6. A charge variant of Fis1 is inserted into microsomes but not into the membrane of mitochondria or lipid vesicles. (A) Radiolabeled precursor of Fis1(TMC-4Q) was incubated for 20 minutes at 25°C with either mitochondria (50 µg protein) or lipid vesicles (33 µg lipids). Further treatment was as described in the legend to Fig. 2D. (B) Radiolabeled precursors of either Fis1-TMC or Fis1(TMC-4Q) were incubated for 20 minutes at 25°C with 50 µg of either mitochondria or microsomes. Further treatment was as described in Fig. 2D.





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