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Fig. 1. Structure and expression of Sdt isoforms. (A) Two sdt transgenes encoding full-length Sdt proteins were used: SdtA [also known as Sdt-MAGUK1 (Bachmann et al., 2001)], and Sdt-B2 (Berger et al., 2007). (B) List of Sdt variants used in this study. All transgene-encoded proteins in A and B carry a FLAG-tag at their N-terminus. (C) Western blot analysis of Sdt-FLAG proteins from the eyes of flies that carry large clones mutant for sdtK85 and express the respective transgene using Rh1GAL4. Anti-FLAG antibody was used to detect Sdt-FLAG proteins. Eyes of flies with large clones mutant for sdtK85 but not expressing Sdt-transgenes were used as control.