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Figure 5


Fig. 5. The divergent L27 domain of Sdt interacts with the L27 domain of PATJ in yeast-two-hybrid assays. (A) Comparison of the two L27 domains of Pals1 with those of Sdt. The yellow boxes indicate conserved hydrophic amino acids required to form helices characteristic for L27 domains. (B) Only Sdt fragments containing the N-terminal L27 domain can bind a portion of PATJ that includes the L27 domain, in the yeast-two-hybrid system. The results of the interactions are tabulated on the right: +, interaction; –, no interaction.





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