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Figure 3


Fig. 3. SNX1 recruitment induces long-range tubules appearing concomitant with vacuolar shrinking. MDCK cells expressing GFP-SNX1 were infected with the SL1344 strain and invasion was imaged live (see supplementary material Movie 2a-d). Selected frames of a region of interest are shown (time in seconds). (A) Phase contrast images. Note the size reduction of vacuoles (arrowhead) at vacuole containing bacteria (arrow). (B) GFP-SNX1 was readily recruited to bacteria and spacious SCVs and labeled long-rage tubules; these spacious vacuole-associated tubules (SVATs) were also occasionally observed to make contact with vesicular SNX1 pools (boxed area). (C-E) Analysis of vacuolar shrinking. (C) The changes of vesicle area (in pixels) per frame of one focal depth from movies of LCI infection assays were analyzed from randomly chosen large light-lucent vesicles (see D) in infected cells expressing GFP-SNX1 (vesicles 6-8, compare E) or non-transfected yet infected cells (1-5). Data are representative for over n=3 independent experiments for each cell type (HeLa or MDCK cells) with over ten cells imaged.





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