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Fig. 5. No substantial recruitment of SNX1 was seen upon infection with a mutant strain that lacked SigD. (A) Cells expressing GFP-SNX1 were infected with a SL1344 strain lacking the inositol phosphatase SigD (
sigD) and infection was imaged for >20 minutes. Selected frames are shown (see also supplementary material Movie 4a,b). Although SNX1-positive tubules from endosomal structures were observed (arrows), there was no pronounced enrichment of GFP-SNX1 at the site of infection (arrowheads). (B) MDCK cells were infected a with wild-type SL1344 or with a SL1344-sigD strain for 15 minutes, then fixed and labeled for endogenous SNX1 (Alexa568, red). Maximum projections of two z-sections (at 488 nm z-separation) are shown. Tubules up to 25 µm could be preserved in control cells (arrows). In SL1344-sigD-infected cells, SNX1 is mainly vesicular with no pronounced recruitment to bacteria or even to very large vacuoles (arrowhead). Scale bar: 10 µm.
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