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Figure 6


Fig. 6. Characterization of infection of SNX1-suppressed cells. Control and SNX1-suppressed cells were infected with SL1344 for 15 minutes, fixed and immunolabeled with anti-SNX1 and treated with DAPI. (A) Additionally, cells were immunolabeled with anti-CI-MPR (Alexa488, green). In SNX1-suppressed cells, the CI-MPR is redistributed to the site of bacterial entry. (B) After removing bacteria, cells were either fixed directly or after further 45 minutes, and additionally labeled with TRITC-phalloidin (red). In SNX1-suppressed cells, S. Typhimurium displays reduced clustering. Scale bar: 10 µm.





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