JCS026427 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

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• Supplemental Figure S1 -

  Fig. S1. Nuclear protein extracts were prepared and subjected to western blot analysis using rabbit polyclonal antibodies against ORC1. The nitrocellulose membrane was incubated with rabbit anti-ORC1 antibody (lane 1) and preimmune serum (lane 2). The antibodies recognize a band at 130 kDa corresponding to the expected size of ORC1, similarly to the previously published molecular weight (Mehra et al., 2005).

• Supplemental Figure S2 -

  Fig. S2. Immunolocalization of ORC1 (red) combined with telomere-adjacent FISH TARE6 probe (green). A fraction of the ORC1 IF signal colocalizes with the telomeres, forming yellow spots in the merged image.