spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Subcellular localization of mutant plectin fragments containing a β-synemin-binding site in transfected C2C12 myoblasts and myotubes. C2C12 myoblasts were transfected with plasmids expressing Myc-tagged plectin Ex 1, CHD{alpha} and PlD-M fragments, and then cultured in differentiation medium. At 60-84 hours post transfection, differentiated myotubes were immunolabeled with antibodies specific for Myc and β-synemin. Localization of β-synemin along SFLSs and throughout the sarcoplasm in a dotted pattern was found in control myotubes doubly immunolabeled with Alxea-Fluor-594-conjugated phallotoxins and anti-β-synemin pAb (A-F). The three mutant plectin fragments more or less colocalized with β-synemin along SFLS (arrows in J-L,P-R,V-X). Arrowheads in V and X indicate sites associated predominantly with β-synemin, but scarcely with PlD-M. Bars, 10 µm.





Right arrow Return to article