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Fig. 8. In vivo interactions of plectin and β-synemin with costameric proteins including DGC. Plectin- and β-synemin-immune complexes were immunoprecipitated from LM lysates by anti-plectin or anti-β-synemin antibodies, respectively. Both the immune complexes contained dystrophin,
-dystrobrevin 1 and
-dystrobrevin 2, meta-vinculin, desmin and actin, including nonmuscle actin, but not
-actinin. In control experiments, control IgG did not significantly coimmunoprecipitate with any proteins.