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Figure 5


Fig. 5. Summary of the 33 different caveolin constructs analysed. (A) EM micrographs of Cav1-null MEFs microinjected with HRP and either HsCav-HA{Delta}3-48, MmCav3-HA{Delta}1-16, CfCav1-HA{Delta}147-178 or HCav-HA49-132 DNA. The cell surface was labelled with CTB-HRP. All the constructs generated caveolae that were similar to endogenous caveolae in wild-type MEFs. Scale bars: 200 nm. (B) Schematic protein alignment illustrating the protein-domain structure and some of the known post translational modifications of HsCav1, HsCav3 and CeCav-a. The scale bar indicates the number of amino acids from the start of the N-terminus of CeCav-a. Pink circles highlight palmitoylated residues, yellow circles mark some of the residues mutated in HsCav3 in muscle diseases. Circles with broken lines and lightly shaded mark non-conserved residues. (C) Summary of the results of EM and light-microscopy experiments from the constructs tested in this study. #, constructs that formed caveolae but were less efficient than wild-type constructs. ^, 20% of cells expressing this construct contained more peripheral structures, but these were judged not to be caveolae by light microscopic methods.





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