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Fig. 6. Live cell, time-lapse photographs of Vero cells transiently expressing RFP-NuMA and infected with F-VP26/GFP at a MOI of 5 PFU/cell (5-12 hours post infection). Individual panels were projected from 20-25 z-axes, confocal sections to represent the total fluorescence in the cell. RFP-NuMA began to decrease in the central parts of the nucleus where the initial accumulation of VP26-GFP was observed (A,B). The solid-lined arrows in B3 and C3 show foci of potential primary enveloped capsids whose egress into the cytoplasm was accompanied by a contortion of the nucleus (C2,D2,E2: dotted-lined arrows) and a pushing-out of the nucleus into the direction of the cytoplasm (C2,D2: fine dotted-lined arrows). Replication compartments (RC, E1) formed most readily in places comparatively void of RFP-NuMA (E3,F3). At late times post infection, the overall RFP-NuMA distribution did not change and remained mostly in a ring-like pattern (G2) while VP26-GFP was dispersed in the cytoplasm (G1). At times later than 15 hours, VP26-GFP in progeny virions was found also in the extracellular space (see supplementary material Movie 1).
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