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Figure 8


Fig. 8. Disruption of G-actin or F-actin does not affect the exclusion of NuMA from replication compartments. HEp-2 cells were infected with HSV-1 at a MOI of 3 PFU/cell and after 1 hour, cultured in the presence of the solvent DMSO (A-D), cytochalasin D (E-H) or latrunculin A (I-L). At 10 hours post infection, the cells were fixed and analysed for NuMA or NuMA/ICP8 localisation. DMSO-treated cells showed the typical decrease in NuMA inside the nucleus as shown by the arrows (A-C) and its exclusion from replication compartments (D). Cytochalasin D, which disrupts F-actin, slightly affected nuclear morphology but exhibited the representative NuMA pattern as shown by the arrows (E-G,H). Latrunculin A, which inhibits G-actin, affected the size of both replication compartments and NuMA-void areas (I-K,L). The arrows in panel I show that decrease of NuMA fluorescence was constricted to much smaller areas. The percentage of cells exhibiting such NuMA relocalisation were quantified (in at least 200 cells) and shown in panels A,E,I.





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