JCS022210 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Immunoblotting of MCD4-cell lysate, total intracellular vesicles (200k vesicles), immunoisolated AQP2-bearing vesicles (I.V. I° and II° Ab) and negative control (I.V. II° Ab alone) were probed with antibodies against Na⁺/K⁺-ATPase. Na⁺/K⁺-ATPase was detected at low levels in both total or immuno-isolated vesicles when compared with the whole lysate (MCD4 lysate). Results are representative of three independent experiments.