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Figure 3


Fig. 3. Immunoisolated AQP2-expressing vesicles contain VAMP2 and VAMP3. Immunoblotting of MCD4 lysate, total intracellular vesicles (200k vesicles), immunoisolated AQP2-bearing vesicles (I° and II° Ab) and negative control (II° Ab alone) probed with antibodies against AQP2, VAMP2 and VAMP3. AQP2, VAMP2 and VAMP3 were expressed and enriched in the immunoisolated vesicles. Results are representative of three independent experiments.





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