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Figure 6


Fig. 6. Effect of SNARE and Munc18 knockdown on AQP2 translocation to the apical surface. Cells silenced for SNARE (left panel) or Munc18 (right panel) isoforms, were subjected to apical surface biotinylation in resting condition (ctr) of after FK stimulation (FK). The amount of biotinylated AQP2 was estimated by western blotting (biot. AQP2). siRNA transfection did not affect the total amount of AQP2 detectable in each sample (tot. AQP2). (C) Densitometric analysis of the 29 kDa biotinylated AQP2 band. Results are expressed as mean values ± s.e. of the values obtained in three independent experiments. The amount of AQP2 detected in mock-transfected cells in resting condition was set as 100%.





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