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Figure 7


Fig. 7. CLSM analysis of AQP2 translocation in MCD4 cells silenced for Munc18b and Munc18c. AQP2 trafficking was analyzed in polarized MCD4 mock-transfected or after transfection with siRNA targeting Munc18b ad Munc18c. Cells were stained with antibodies against AQP2 and counterstained with WGA-TRITC (red). Confocal scans were taken in the yz plane. In mock transfected and in Munc18c-silenced cells AQP2 was found mainly associated with subapical vesicles and translocated towards the apical membrane after FK treatment. Munc18b knockdown produced a clear accumulation of AQP2 at the apical plasma membrane, even without FK stimulation.





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