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Figure 2


Fig. 2. Spatial characteristics of the AR in human sperm. (A) The acrosome of each acrosome-reacting sperm was divided into three regions: (1) anterior, (2) mid-section and (3) posterior. (B) Histogram showing the initial site of uptake of Alexa-488–SBTI (green) and fluorescent anti-CD46 (red) in 72 acrosome-reacted sperm from five experiments. Bars show s.e.m. (C) Image series showing uptake of Alexa-488–SBTI (green) in three different sperm (i-iii); numbers represent time in seconds. (D) Image series showing uptake of fluorescent anti-CD46 (red) in three different sperm (i-iii); numbers represent time in minutes. Scale bars: 2 µm.





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