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Fig. 7. The LOX-1 cytoplasmic domain contains a novel aspartate-based endocytic motif. (A) Multiple sequence alignment of mammalian LOX-1 cytoplasmic domains. Completely conserved amino acid residues are indicated by an asterisk, conservative amino acid substitutions are indicated by a colon and predominantly conserved amino acid substitutions are indicated by a full stop. LOX-1 wild-type and mutant proteins were expressed and OxLDL uptake monitored (see Materials and Methods) by microscopy (A) and quantified (B). (A) Fixed cells were labelled with mouse anti-FLAG and FITC-conjugated anti-mouse IgG antibodies. Arrows indicates areas of clustered LOX-1 and OxLDL ligand at the plasma membrane. (C) The uptake of labelled OxLDL in LOX-1-transfected HeLa cells were quantified (see Materials and Methods). The percentage of transfected cells with internalised OxLDL was counted (n=3 separate experiments, 50 cells from each experiment, mean ± s.e.m.). Comparison with control LOX-1 WT was used to calculate P values, *P<0.01.
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