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Figure 4


Fig. 4. Immunostaining of wild-type, hop2A{Delta} and spo11{Delta} meiotic MICs. (A-C) Cna1 staining of centromeres demonstrates that, starting from a clustered arrangement and passing through a stage of dispersal, centromeres occupy a small area in the tip of the elongated MIC in wild type and in the hop2A{Delta} mutant. In the spo11{Delta} mutant they remain dispersed. The DSB marker {gamma}-H2A.X delineates MICs in wild type (D) and in the hop2A{Delta} mutant (E), and in the spo11{Delta} mutant only after DSB induction by cisplatin (F). The recombination marker Rad51p forms foci in the elongating MICs of wild type (G) and the hop2A{Delta} mutant (H), and displays a uniform nuclear distribution at later stages. It does not form foci in the spo11{Delta} mutant (I). Scale bar: 10 µm.





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