spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. The differential degradation of the Na+/K+-ATPase subunits in the plasma membrane. HeLa cells at 80% confluence were pulse-labeled with membrane-impermeable biotinylation reagent (sulfo-NHS-SS-biotin) at 4°C for 1 hour, placed back in DMEM and incubated at 37°C for 0, 1, 3 or 5 hours in the absence or presence of inhibitors for degradation pathways. At the end of the chase period, the cells were harvested before (–GSH) and after (+GSH) treatment with glutathione to remove biotin moieties remaining on the cell surface. The labeled proteins were purified with streptavidin-agarose and analyzed by immunoblotting using antibodies against {alpha}1-, β1- and β3-subunits. (A) A typical result of the immunoblot analyses. (B) Quantitative analyses of the immunoblot. The results from at least three independent experiments are summarized.





Right arrow Return to article