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Figure 1


Fig. 1. Subcellular distributions of TERT C-terminal polypeptide sequences expressed in HeLa cells. (A) Schematic presentation of full-length TERT and its indicated C-terminal polypeptide fragments used in this study. (B) An example of the subcellular distribution patterns of different TERT C-terminal fragments in transiently transfected HeLa cells (refer to text for detailed description). 400x magnification. Green, signal for ectopically expressed GFP-tagged fragments; red, signal for the co-expressed RFP-B23; blue, DAPI nuclear staining. The merge shows the overlay images of GFP-TERT fragments and the co-expressed RFP-B23 nucleolar protein within nuclei. The percentages of cells with the represented GFP staining patterns in the corresponding transfected cell populations are shown to the right (>400 expressing cells counted for each group).





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