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Figure 4


Fig. 4. Mutational inactivation of the C-terminal NTS of TERT abrogates the DNA-damage-induced nucleolar localization of TERT. Wild-type GFP-TERT and the mutant GFP–TERT-3A were stably expressed in the indicated human cancer cell lines through lentiviral infection. (A) Treatment with 50 µM etoposide for 6 hours stimulated the expressed GFP-TERT to translocate from the nucleoplasm to nucleoli in all tested stable cancer cell lines. (B) The same etoposide treatment did not alter the intranuclear distribution of the expressed GFP-TERT mutant in all tested stable cells. Green represents the fluorescence signal for expressed GFP-TERT or GFP–TERT-3A in the corresponding stable cells before and after etoposide treatment. Arrows indicate the nucleolar structures observed under the optic microscope. 400x magnification. The percentage of cells with the represented GFP staining patterns in the corresponding transfected cell populations are shown on the right (>400 expressing cells counted for each group).





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