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Fig. 7. ROCK inhibition abrogates the dominant effect of the phospho-mimicking annexin A2 mutant. (A) BHK-IR cells were transfected with the phospho-mimicking mutant Anxa2-Y23E-GFP and either left untreated or were treated for 1 hour with 20 µM of the ROCK inhibitor Y27632. Cells were then fixed and stained for F-actin. Note the induction of the `insulin-responsive' actin phenotype in Anxa2-Y23E-GFP expressing cells (lower panels), which is abrogated by ROCK inhibition (upper panels). Scale bars, 10 µm. (B) Several hundred cells expressing Anxa2-Y23E-GFP were inspected and the relative number of cells showing the normal F-actin distribution (rescued phenotype) was quantified for experiments carried out in the presence (+) or absence (–) of Y27632. **P<0.0005.
