JCS024703 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The distribution patterns of GFP-Bax in TNFα/CHX-induced apoptotic HeLa cells and UV-induced apoptotic PtK2 and U2OS cells. (A-L) Sample records of GFP-Bax distribution (A-D), Mitotracker distribution (E-H) and merged images (I-L) in TNFα/CHX-induced apoptotic HeLa cells. (M-T) Sample records of GFP-Bax distribution in UV-induced apoptotic PtK2 cells (M-P) and U2OS cells (Q-T).

• Supplemental Figure S2 -

  Fig. S2. The relationship between Bak distribution and the decrease of mitochondrial membrane potential during UV-induced apoptosis in HeLa cells. (A) A sample record of the distribution of YFP-Bak, which started to form small complexes in a few mitochondria of the cell. (B) The distribution of Mitotracker of the same cell. (C) The merged image of YFP-Bak and Mitotracker in A and B.

• Movie 1 -

  Movie 1. The temporal relationship between Bax distribution (GFP-Bax, left panel) and the decrease of mitochondria membrane potential (Mitotracker, right panel) during UV-induced apoptosis in HeLa cells.

• Movie 2 -

  Movie 2. The temporal relationship between Cyt c distribution (Cyt c-GFP, left panel) and the decrease of mitochondria membrane potential (Mitotracker, right panel) during UV-induced apoptosis in HeLa cells.

• Movie 3 -

  Movie 3. The temporal relationship between Smac distribution (Smac-YFP, left panel) and the decrease of mitochondria membrane potential (Mitotracker, right panel) during UV-induced apoptosis in HeLa cells.

• Movie 4 -

  Movie 4. The temporal relationship between Bax distribution (CFP-Bax, left panel) and Smac distribution (Smac-YFP, right panel) during UV-induced apoptosis in HeLa cells.