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Figure 3


Fig. 3. Time-dependent measurement of GFP-Bax distribution during UV-induced apoptosis in live HeLa cells. (A-H) Sample records from a time-series of confocal measurements of GFP-Bax distribution (left) and Mitotracker distribution (right) on two live HeLa cells. The cells beginning to form small complexes of GFP-Bax are indicated with arrowheads. Scale bar: 10 µm. (I) Quantitative analysis of the translocation of GFP-Bax and Mitotracker fluorescence intensity change of Cell 2 shown in A-H. (J) Analysed results of another imaging measurement showing that the release of Cyt c from mitochondria correlates with the reduction of Mitotracker fluorescence intensity.





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