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Figure 7


Fig. 7. ERK1/2 mediates the effect of EGF on TGFβ1-mediated Smad-driven promoter activity. OKF6 (A) and UMSCC1 (B) cells were transfected with 1 µg of SBE-4 luciferase construct, and equal numbers of cells were plated overnight, serum starved for 8 hours, and then treated with either DMSO or U0126 for 30 minutes prior to treatment with EGF (20 ng/ml). The samples were then treated with TGFβ1 (10 ng/ml) 30 minutes later for additional 24 hours. The samples were lysed, luciferase activity was quantified, and values normalized to DMSO-treated untreated samples arbitrarily set at 1.0. *P<0.05, significantly different from the DMSO,EGF(–),TGF-b1(+) samples. The results are representative of at least three independent experiments.





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