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Fig. 9. EGF attenuates TGFβ1-mediated migration of oral keratinocytes, but increases TGFβ1-mediated migration of OSCC cells. Serum-starved OKF6 and UMSCC1 cells were untreated, treated with EGF (20 ng/ml) or TGFβ1 (10 ng/ml), or pre-treated with EGF (20 ng/ml) for 30 minutes and then treated with TGFβ1 for 24 hours. The cells were then added to transwell chambers that had been coated with type I collagen, for additional 24 hours in the presence or absence of TGFβ1 and/or EGF. Nonmigratory cells were removed from upper chamber, and migrating cells were counted. *P<0.05, significantly different from the EGF(–),TGF-b1(+) OKF6 cells. The results are representative of at least two independent experiments.