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Fig. 1. Plants deficient for SMG7 exhibit growth defects and sterility. (A) Schematic representation of the Arabidopsis SMG7 gene with the indicated T-DNA insertions in the smg7-1, smg7-3 and smg7-5 alleles (see supplementary material Fig. S2 for the molecular structure of the insertions). White boxes represent exons. (B) Northern blot hybridization analysis showing the expression of the SMG7 gene in the indicated tissues. Full-length SMG7 cDNA was used as a probe. RNA loading was monitored by hybridization with a probe specific for the ACTIN2 (ACT2) gene. (C) Expression of the truncated transcripts from the SMG7 loci in smg7-1 and smg7-3 mutants. A molecular-weight marker is indicated. (D-H) Vegetative-growth phenotypes. (D) A silique from a plant heterozygous for the smg7-5 mutation with aborted seeds (indicated by arrowheads). (E) smg7-1 mutant plant 4 weeks after germination. Scale bar: 0.5 cm. (F) smg7-3 mutant plant germinated and grown for 4 weeks in soil. A control wild-type (wt) plant is indicated on the left. (G) 8-week-old smg7-1 mutant, which was grown for 3 weeks on agar before being transferred to soil. A 4-week-old wild-type plant is shown on the left. (H) Inflorescence bolts from wild-type and smg7-1 mutant plants. (I) Viable pollen grains in wild-type anthers have a dark-blue appearance after Alexander staining. Anthers of smg7 mutants are usually empty or contain only a few nonviable pollen-like structures that stain green. Scale bars: 0.1 mm.
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