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Figure 3


Fig. 3. Aberrant meiosis in SMG7-deficient PMCs. (A-I) Meiosis in wild-type PMCs. (J-T) Meiosis in smg7-1 PMCs. (A,J) Pachytene, (B,K) metaphase I, (C,L) anaphase I, (D,M) telophase I, (E,N) metaphase II, (F,O) anaphase II. (G-I) Telophase II (G) in wild-type PMCs is followed by cytokinesis that results in the formation of tetrads (H) and microspores (I). (P,Q) smg7 mutants fail to decondense chromosomes during the second meiotic division, resulting in `irregular anaphase II' (P) and `scattered chromatids' (Q) meiocytes. (R-T) Decondensed chromosomes tend to cluster (R,S), forming polyads (T). (U-W) The distribution of chromosome 4 during late meiosis II was investigated by 3D-FISH with a chromosome-4-specific probe (green) in wild-type (U) and smg7-1 (V,W) plants. (U) Regular separation of chromosome 4 in A2. (V) Bilateral distribution of chromosome 4 during `irregular A2'. The broken line separates the products of the first meiotic division. (W) The clustering of chromosome 4 in the `scattered chromatids' meiocyte indicates random distribution of chromosomes at this stage. A-C and J-L were prepared by spreading PMCs fixed in ethanol:acetic acid (3:1; v/v) for better chromosome morphology. To preserve the 3D organization of the chromosomes in late meiotic PMCs (D-I, M-T and the 3D-FISH preparations U-W), cells were prepared from floral buds fixed in 4% paraformaldehyde. Scale bars: 5 µm.





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