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Fig. 3. PPAR ${gamma}$ activation increases p16 protein levels in 2BS and WI-38 cells. 2BS and WI-38 cells were transfected with the expression plasmids pcDNA3.1 (vector), pcDNA3.1-PPAR ${gamma}$ (PPAR ${gamma}$ ), pSilencer 2.1-U6 neo (RNAi vector) or pSilencer-PPAR ${gamma}$ (siPPAR ${gamma}$ ) and treated, as indicated, with 20 µM troglitazone or DMSO (vehicle) for 72 hours. (A) Western blot analysis of PPAR ${gamma}$ and p16^INK4 expression in vector-transfected or PPAR ${gamma}$ -transfected cells. (B) Western blot analysis of PPAR ${gamma}$ and p16^INK4 expression in RNAi-vector-transfected or siPPAR ${gamma}$ -transfected cells. Western blotting was performed using specific antibodies against PPAR ${gamma}$ and p16^INK4 as indicated. The β-actin lane served as a loading control.

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