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Figure 6


Fig. 6. H2O2 induces Src-kinase activation. C2 myoblasts were incubated with 10 µm H2O2 for 15 minutes. Immunoblots were probed for pY418-Src, striped then re-probed for total Src. A sample blot is shown. The graph represents the fold change in Src activation compared with control (pY418-Src/total Src). H2O2 incubation increased Src phosphorylation/activation 1.8 times compared with control cells. Such an increase was completely blocked when cells were incubated with PP2 prior to H2O2. Graph represents mean ± s.e.m. of four dishes per group from two independent experiments. *P<0.05; ***P<0.001.





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