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Figure 2


Fig. 2. FXIIIA-stimulated expression of type X collagen is dependent upon TG2. Assessment of TG2- and FXIIIA-knockout mouse cells. Primary knee chondrocytes were removed from F13a1+/+, F13a1 –/–, Tgm2+/+ and Tgm2–/– mice. After two weeks in culture, aliquots of 5x103 cells in Medium A were stimulated for 5 days with 10 nM ATRA, 10 ng/ml CXCL8 or 100 ng/ml of sTG2 or sFXIIIA, and then type X collagen was examined by SDS-PAGE and western blotting, as described in the Materials and Methods. Representative of three experiments.





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