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Fig. 3. (A) FXIIIA. Site-directed FXIIIA mutations generated for structural analysis examination. Depicted are features of the primary structure of WT FXIIIA and the site-directed FXIIIA mutations generated and studied, listed her as 1-3. (B) Type X collagen. Aliquots of human articular chondrocytes (105 cells) were incubated with 100 ng/ml of each recombinant protein in medium A for 72 hours, and type X collagen assessed in cell lysates by SDS-PAGE and western blotting. Representative of three donors in three separate experiments (n=9).