spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Recombinant FXIIIA engages the {alpha}1-integrin subunit in human chondrocytes. (A) Western blot. Aliquots of 105 human articular chondrocytes were starved for 2 hours in serum-free DMEM and then incubated for the indicated times with 100 ng/ml sTG2 or sFXIIIA. The cells were washed prior to lysis and then examined for the presence of the Xpress epitope on the recombinant proteins by SDS-PAGE and western blotting. (B) Integrin immunoprecipitation. Aliquots of 106 human articular chondrocytes were incubated for 3 days in medium A containing 100 ng/ml of sTG2 or sFXIIIA where indicated. Cell lysates (200 µg protein) were immunoprecipitated using 1 µg/ml of {alpha}1- (clone TS2/7), {alpha}2-, {alpha}5- or {alpha}6-integrin-subunit-specific antibody, and precipitated proteins were analyzed for the Xpress tag or each respective {alpha}-integrin subunit by western blotting. Representative of three separate experiments using three different donors.





Right arrow Return to article