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Figure 6


Fig. 6. Coexpression of CD9 and TGF{alpha} confers changes in actin stress fibers and activities of Rho family of small GTPases that can be rescued by blocking the EGFR activation. (A) MDCK cells expressing CD9, TGF{alpha} or CD9 and TGF{alpha} were stained with Alexa-Fluor-594–phalloidin (red) or by immunofluorescence for paxillin (green), and analyzed by confocal microscopy. The last two images (top and bottom) show MDCK cells expressing CD9 and TGF{alpha} in the presence of a blocking EGFR antibody. (B) The same cells as shown in A were evaluated for the level of activated, GTP-bound forms of the small GTPases RhoA, Cdc42 or Rac1. (C) RhoA and Rac1 GTPases activities were analyzed in the presence of the EGFR inhibitor AG1478. (D) CD9 expression does not change the distribution of actin stress fibers or focal adhesions when expressed with transmembrane TGF{alpha} that lacks the TGF{alpha} core domain. MDCK cells that express TGF{alpha}{Delta}E, or TGF{alpha}{Delta}E and CD9 together were stained using phalloidin (red) or paxillin (green) and analyzed by confocal immunofluorescence microscopy.





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