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Figure 6


Fig. 6. Structural consequences of mechanical (A,B) and chemical (C,D) treatment of the capsids. Overall shape and dimensions of the majority of the capsids remains intact although the height of several capsids is decreased. AFM was performed at the loading force of 2 nN. A and B as well as C and D are paired experiments, although A and B represent the same area whereas C and D represent different areas of the same sample. (E) Measured stiffness values of intact, mechanically damaged and GuHCl-treated capsids. DNA contribution to the overall stiffness of the nucleocapsid evaluated by mechanical probing of empty capsids appears to be significant (data are shown as mean ± s.e.m.). The AFM images are 3.5x3.5 µm.





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