spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. FCPT decreased spindle-pole microtubule density. (A) FCPT-treated (200 µM) Xenopus egg-extract spindles did not collapse, whereas STLC (non-competitive kinesin-5 inhibitor)-treated spindles collapsed within 20 minutes. Fixed images of different spindles at time points up to 20 minutes showing tubulin (red), NuMA (green) and DNA (blue) are shown. The spindle-pole marker NuMA remained localized to the spindle pole in the presence of FCPT. (B) FCPT-treated Xenopus egg-extract spindles maintained a constant length of about 35 µm, whereas STLC-treated spindles collapsed to approximately 0 µm over 20 minutes. Error bars, s.e.m.; n=6 for each time point. (C) The ratio of the fluorescence of the spindle-poles:mid-spindle decreased from approximately 0.9 to approximately 0.6 over 40 minutes. Error bars, s.e.m.; n=6 for each time point. (D) Spindles assembled in the presence of FCPT are elongated and asymmetric, compared with controls, which are bipolar. Fixed images of spindles showing tubulin (red), NuMA (green) and DNA (blue) are shown. NuMA was diffusely localized on FCPT-treated spindles. Scale bars: 10 µm.





Right arrow Return to article