spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Knockdown of TRPC6 impairs dendritic growth. (A) Effectiveness and specificity of TRPC6-shRNAi. The two upper blots are total lysates of hippocampal cultures transfected with the shRNAi constructs targeting rat TRPC6 (shRNA6i-1 or shRNA6i-2) and control shRNAi construct targeting firefly luciferase (pPRIME-FF3) 96 hours after transfection using antibodies against TRPC6 and tubulin. The three lower blots show total lysates of HEK293 cells transfected with rat TRPC5 or human TRPC6 and shTRPC6i-1 (targeting both rat and human TRPC6) or shTRPC6i-2 (targeting only rat TRPC6) 48 hours after transfection detected using the indicated antibodies. (B) Representative images of the neurons transfected at 3 DIV for 4 days with control vector, shTRPC6i-2, shTRPC6i-1 plus human TRPC6 or shTRPC6i-2 plus human TRPC6. Scale bar: 20 µm. (C,D) Quantification of total dendritic tips (C) and total dendritic length (D) of the neurons shown in B. **P<0.01 versus control (ctrl) or shTRPC6i-2 (shC6i-2) plus hTRPC6.





Right arrow Return to article