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Figure 1


Fig. 1. Inhibitors of PKC but not PKB activity block budding of PCTVs. (A) PCTV-budding activity using native cytosol (NC) or 170 nM the PKB inhibitor Akti-1/2 (Akti viii). (B) PCTV-budding activity in response to increasing doses of calphostin C as indicated. PCTV-budding activity was determined after pre-incubation of the cytosol used with the indicated doses of calphostin C (µg) in the light. The data are shown as a percentage of the activity obtained on incubation of intestinal ER with native cytosol. (C) PCTV-budding activity as a percentage of ER incubated using native cytosol (NC). ER was incubated using cytosol pre-incubated with calphostin C (4 µg) in the dark (Dark) or calphostin C (4 µg) exposed to light (Light). P values indicate differences between calphostin C incubated in the light vs incubation in either the dark or cytosol without calphostin C (NC). Data are the mean ± s.e.m., n=4.





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