spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. SCA14 mutations located in C1B affect C1B translocation kinetics upon phorbol ester stimulation. (A) Confocal images of HeLa cells cotransfected with C1B-GFP and SCA14 mutant C1B-RFP. PMA activation induces irreversible translocation of C1B-GFP and C1B G118D-RFP to the plasma membrane. By contrast, the V138E and C142S C1B-RFP did not respond to PMA activation. Images shown were recorded prior to (0) and 200 seconds after addition of 400 nM PMA. Scale bars: 10 µm. (B) Translocation analysis of cotransfected C1B-GFP and mutant G118D, V138E or C142S C1B-RFP showed that the V138E and C142S mutations impair phorbol ester (400 nM PMA)-induced membrane translocation. Each of the traces in B and C represent mean ± s.e.m. of 4-6 cells from at least three independent experiments.





Right arrow Return to article